Cell

Part:BBa_V1004

Designed by: n/a   Group: Arkin Lab   (2004-07-14)

DH10B

Like the Top10 cell strain, this strain is also an Invitrogen product

Usage and Biology

  • suitable for cloning methylated cytosine or adenine containing DNA
  • an MC1061 derivative (Casadaban80). Prepare cells for chemical transformation with CCMB80 buffer
  • blue/white selection
  • Promega also lists deoR (but see Top10 cell genotype)
  • Streptomycin resistant
  • Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
Parameters
genotypeF- endA1 recA1 galU galK deoR nupG rpsL ΔlacX74 Φ80lacZΔM15 araD139 Δ(ara,leu)7697 mcrA Δ(mrr-hsdRMS-mcrBC) λ-